Flow Cytometry and cell Sorting Facility

The flow cytometers that are managed within the facility offer various possibilities. Based on the objective of the experiments, a device that answers the research question is selected in consultation with the researcher.
For example, if a viability assay is being performed and the readout will be one or two targets, low-end instruments can be chosen. For more extensive experiments, i.e., with more fluorescently labeled markers, there are several options, including for sorting cells from mixed populations.

The options for each device are explained below.

Analyzers:
Beckman Coulter, Cytoflex S (2 available)

• Benchtop analyzer with4 lasers, 13 channels
• 96 well plates of tubes
• 3-training session program
• MLII-level in in a Biosafety cabinet

Beckman Coulter Cytoflex LX (1 available)

• Benchtop analyzer with 6 lasers, 21 channels
• 96 well plates of tubes
• 3-training session program
• MLI-level on bench

Cytek Aurora (1 available)

• Benchtop spectral analyzer met 5 lasers and 62 channels
• 96 of 384 well plates and tubes
• 3-training session program
• MLI-level in a biosafety cabinet

Sony ID 70000 (1 available)

• Benchtop spectral analyzer met 6 lasers and 182 channels
• 96 of 384 well plates and tubes
• 3-training session program
• MLI-level on bench

BD FACS Symphony A1 (1 available)

• Benchtop analyzer with 4 lasers, 18 channels
• 96 and 384 well plates or tubes
• 3-training session program
• MLI-level on a bench

BD FACS Symphony A3 (1 available)

• Benchtop analyzer with 5 lasers, 30 channels
• 96 and 384 well plates or tubes
• 3-training session program
• MLI-level om bench

Cell sorters
Sony SH800s (2 available)

• Benchtop cell sorter co-linear alignment with 4 lasers, 6 channels
• Sort into 96 and 384 well plates or tubes.
• Collection of 2 populations
• 3Chip choice is 70, 100 or 130uM
• A 3-training session program
• Both instruments on MLII level in a biosafety cabinet

BD FACS Aria fusion (1 available)

• Cell sorter with 5 lasers, 18 channels
• Sort into 96 and 384 well plates of tubes.
• Collection of 4 populations
• Nozzle sizes; 70, 85, 100 and 130uM
• A 3-training session program
• MLI-level in a biosafety cabinet