Centre for Flow Cytometry and Cell Sorting (CFC)

The Centre for Flow Cytometry and Cell Sorting at the Faculty of Veterinary Medicine provides expertise in single cell measurement and sorting since 1998. The facility supports a wide range of analyses and purifications, including immune and stem cells, sperm cells, chromosomes, bacteria, hybridomas for monoclonal antibody production, single-cell sorting for cloning or PCR analysis, fluorescent reporter–based cell selection and rare event purification. We have accumulated expertise in small particle flow cytometric analysis guiding novel developments in the field and acquiring high-sensitivity equipment capable of measuring particles as small as 40 nm in size. We have a strong track record of translating complex experimental demands into successful solutions through close collaboration with research groups, instrument manufacturers and continuous technical development.

Available Equipment:

Cell Sorting
BD Influx Cell Sorter – Equipped with 5 lasers (355 nm, 405 nm, 457 nm, 488 nm, 561 nm, 635 nm) enabling up to 21 fluorescent parameters. This jet-in-air system supports up to six-way sorting into a variety of tubes and plate formats.
BD FACSAria Fusion – Equipped with 4 lasers (405 nm, 488 nm, 561 nm, 635 nm) for up to 14 fluorescent parameters. This fixed-alignment cuvette flow cell sorter is integrated into a biosafety cabinet and supports four-way sorting into tubes and multiple plate formats.

Cell Analysis
CytoFLEX LX Analyzer – Equipped with 6 lasers (375 nm, 405 nm, 488 nm, 561 nm, 635 nm, 808 nm), allowing up to 21 fluorescent parameters. Supports both tube- and plate-based measurements.
Cytek Aurora ESP Spectral Analyzer – Equipped with 3 lasers (405 nm, 488 nm, 635 nm), supporting up to 25 fluorescent parameters. Capable of both tube- and plate-based measurements. Includes a feature to measure and subtract intrinsic cellular autofluorescence.

Small Particle Analysis (Extracellular Vesicles, Nanoparticles, etc.)
CytoFLEX Nano – Dedicated to particles smaller than 1 µm. Offers VSSC detection with reduced background levels. Equipped with 4 lasers (405 nm, 488 nm, 561 nm, 638 nm) providing 6 fluorescent parameters and 6 light scatter parameters. Supports tube-based measurements.
Cytek Aurora ESP – Equipped with a high-sensitivity secondary SSC detection channel for improved small particle analysis while retaining capability for larger particles.

Data Analysis
Three dedicated workstations with FlowJo licenses for routine downstream analysis.
High-end workstation supporting FlowJo, SpectroFlo, R Studio, FCMpass, and other software for advanced, high-dimensional data pipelines.